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. Author manuscript; available in PMC: 2009 Mar 10.
Published in final edited form as: Cell. 2008 Jun 13;133(6):1032–1042. doi: 10.1016/j.cell.2008.05.039

Figure 3. Mad2 and Ncd Are Required for the Suppression of Spindle Multipolarity.

Figure 3

(A–C) Immunostaining of mad2,SAKOE mitotic neuroblasts with D-PLP (left, green in merged panels), α-tubulin (2nd panel, red in 5th panel), Cnn (3rd panel, red in 6th panel), and DNA (blue in merged panels). For comparison with WT controls see Figures 2A and 2E. Tripolar anaphases (A), anaphases with lagging chromatids (arrow in B), and polyploid cells (C) are often seen in mad2,-SAKOE brains but very rarely in SAKOE neuroblasts (see Table 1). Note that mad2,SAKOE neuroblasts can also contain much larger numbers of extra centrosomes than is ever seen in SAKOE cells (~30 in the cell shown here).

(D and E) Immunostaining of ncd,SAKOE mitotic neuroblasts with D-PLP (left panel, green in merged panels), α-tubulin (2nd panel, red in 4th panel), Cnn (3rd panel, red in 5th panel), and DNA (blue in merged panels). For comparison with WT control neuroblasts see Figures 2C and 2E. In ncd,SAKOE neuroblasts multipolar metaphases can be detected (D) but almost all anaphases are bipolar (E). Scale bar = 10 µm.

(F) A graph showing the percentage of WT (white bars), SAKOE (red bars), mad2 (light blue), mad2,-SAKOE (dark blue), ncd (bright green), and ncd,SAKOE (dark green) pupae that formed between the 5th and 10th days of development. Note that the WT and SAKOE data shown here are the same as that shown in Figure 1A, as these experiments were performed at the same time.