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. 2009 Mar 16;4(3):e4875. doi: 10.1371/journal.pone.0004875

Figure 4. Optimum HIV replication requires caspase 8.

Figure 4

(A) Jurkat T cells (squares) or I9.2 cells (diamonds) were infected with HIV IIIb, and analyzed daily for viability (red) and for P24 production (blue). (B) Two days following infection, cells were harvested, and DNA extracted. The DNA was assayed for HIV Nef by PCR. (C) Non-transfected Jurkat T cells or I9.2 cells transfected with control vector or procaspase 8, and stimulated with TNF or CD3/CD28 as indicated were analyzed for HIV LTR driven expression normalized to Renilla.