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A. Immunoblot of Gef1. The Gef1 Ab identifies a ca. 100 kDa band (PSec7) from Paramecium, and an ortholog (Gef1) in Tetrahymena cell and ciliary membrane fractions. The Sec7 motif, which delineates a function in guanine nucleotide exchange, is found in both proteins (Grey letters indicate similarity). B. Co-immunoprecipitation of Kin5 and Gef1. 1: Kin5 immunoprecipitate probed with Gef1 Ab. 2: Gef1 immunoprecipitate probed with Kin5 Ab.
