Figure 2.

Perfusion pressures maintained at physiological levels with use of agarose gel. a) Ex vivo perfusion pressure curve demonstrating initial perfusion period with high pressures. After addition of warm PBS-agarose matrix, perfusion pressures decrease (single arrow). Solidifying agarose caused a transient increase in pressures (double arrows), followed by a gradual decrease with the extended perfusion period. b) EMBs are cast into a PBS-agarose matrix at the start of the perfusion period. c) Following extended perfusion, there is little leakage or edema, and flow is preserved through the vascular bed.