Figure 4.

Artificial oxygen carrier augments EMB viability. a) Ex vivo tissue oxygenation with DMEM perfusate only and supplementation with oxyglobin (blue shading indicates physiological oxygenation in vivo). b) Oxygenation (bottom) was directly related to flow and perfusion pressures (top). c) NBT viability staining of EMBs perfused for 24 h with DMEM perfusate alone compared to HBOC supplementation. There was greater EMB viability in the HBOC-supplemented perfused EMBs compared to those perfused with DMEM alone; *P < 0.001. d) Percentage EMB survival at PRD7 after 24-h perfusion with DMEM perfusate alone compared to HBOC supplementation. Seventy-five percent of EMBs subjected to 24-h perfusion with HBOC survived as compared to 50% of those perfused with DMEM alone.