Figure 6.

Reversal of chemokine-dependent conformational changes in CXCR4 homodimers. Type 231 cells stably expressing CXCR4 homodimer reporters were treated for 15 min with various concentrations of CXCL12 before quantifying bioluminescence (no washout). Parallel wells of cells were treated identically with CXCL12, washed with PBS, and then incubated for 30 min without chemokine (30 min washout). Data were normalized to bioluminescence in control cells not treated with chemokine (NT) and graphed as means ± se (n=4/condition, representative of two independent experiments).