Figure 7.

Effect of PSGL-1 blockade on retinal expression of inflammatory mediators. A) TNF-α and MCP-1 expression in retinal tissue of EIU mice. Retinal mRNA expression of TNF-α and MCP-1 in control (CTR) and EIU mice, treated with PSGL-1-neutralizing mAb (Ab) or isotype-matched control IgG (Iso), 6 and 24 h after LPS injection. B, C) Semiquantitative analysis showing the relative amount of TNF-α (B) and MCP-1 mRNA expression (C) as a ratio of the gene product relative to the expression of the GAPDH (arbitrary units). Each data point represents an average of 6 to 10 experiments. *P < 0.05; **P < 0.01.