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. 2009 Mar;133(3):295–305. doi: 10.1085/jgp.200810125

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© 2009 Cheng et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — KirBac1.1 voltage-clamped currents are potassium selective and blocked by barium. Inside-out patches were excised from giant liposomes reconstituted with (A) no protein or (B) WT KirBac1.1. (A) Representative currents from an inside-out patch from giant liposomes without KirBac1.1 protein. The pipette was filled with 158 mM K⁺ and the bath with the indicated solutions. The voltage was held at −50 mV and then stepped from −100 to +100 mV at 10-mV increments. (B) Representative currents from giant liposomes reconstituted with KirBac1.1 as in A. (C) Plot of normalized current versus voltage for WT KirBac1.1 in the solutions shown in B (n = 6 ± SEM). (D) Relative conductance versus voltage plot of KirBac1.1 block in 1 mM Ba²⁺ (n = 6 ± SEM). Fitting with a Boltzmann function gives the following parameter values: V_1/2 = 25 mV, δz = 1.5, residual G_REL = 0.08, scaling factor = 0.9.