TABLE II.

Simulations of Effect of Antechamber Binding and Inactivation on the Time Course of Trypsin-mediated Removal of Inactivation in Accordance with Scheme 1

Po is the open probability defined by the closed-open equilibrium constant, L. k_i is the forward rate of block by a single inactivation domain, with k_r, the unblocking rate. B_i reflects the fractional occupancy of the inactivation binding site conditions of high Po and saturation of the antechamber site. p_f and p_r are rates of binding and unbinding of an N terminal to the proposed antechamber site, with B_a reflecting the fractional occupancy of the antechamber. k_t is the rate of digestion of a single N terminus. τ_d and n are the digestion time constant and power terms from fits of Eq. 1 to the digestion time course simulated with the given parameters. Because Eq. 1 is not an explicit derivation of the model used for simulation, τ_d and n are not exact values, but the confidence limits on the fitted values were typically <1–4%.