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. 2009 Mar 20;4(3):e4861. doi: 10.1371/journal.pone.0004861

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Cheng et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Validation of PPP1R11 antibody was performed as follows: recombinant PPP1R11 and testis protein extracts were separated by SDS-PAGE followed by western blot analysis with affinity purified rabbit polyclonal anti-PPP1R11. Size markers (left) were derived from β-Galactosidase (116-kDa), Phosphorylase b (97.4-kDa), Albumin (66-kDa), Glutamic dehydrogenase (55-kDa), Ovalbumin (45-kDa), Glyceraldehyde-3-phosphate dehydrogenase (36-kDa), Carbonic anhydrase (29-kDa), and Soybean trypsin inhibitor (20-kDa). B. Soluble protein extracts from testis and somatic tissues were separated by SDS-PAGE followed by western blot analysis with the validated affinity purified PPP1R11 antibody, and the same blot was stripped and reprobed with anti-actin used as a loading control.