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. 2009 Mar 20;4(3):e4861. doi: 10.1371/journal.pone.0004861

Figure 2. Sds22, PPP1R11, and PP1γ2 are bound to each other in crude testis protein extracts.

Figure 2

A. The recombinant proteins GST-PPP1R11 and control GST were incubated with testis cell lysates in the presence of Glutathione-Sepharose beads. The eluted proteins and testis extracts alone were resolved by SDS-PAGE and subjected to western blot analysis with anti-Sds22 and anti-PP1γ2 antibodies. B. Testis protein extracts and buffer controls were incubated with anti-PP1γ2, anti-PPP1R11, or preimmune serum immobilized on Protein G-Sepharose-4 beads, as indicated at the top of the figure. The immunoprecipitates were separated by SDS-PAGE and immunoblotted for the proteins indicated at the bottom of the figure. (ND: not done).