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. 2009 Mar 20;4(3):e4861. doi: 10.1371/journal.pone.0004861

Figure 3. PPP1R11, Sds22 and PP1γ2 co-elute during chromatographic purification of testis extracts.

Figure 3

Western blot analysis of purified column fractions shows a consistent co-elution pattern of testicular PP1γ2, Sds22, and PPP1R11 through a series of chromatographic media. A. DEAE chromatography showing PP1γ2, Sds22, and PPP1R11 co-eluting primarily in fractions A3 and A4. B. A3 and A4 fractions from the DEAE column were pooled and purified by MonoQ column fractionation. PP1γ2, Sds22, and PPP1R11 co-eluted in fractions B8–11. C. B8–B11 fractions from the MonoQ column were pooled and purified by Superose 6 column chromatography, from which PP1γ2, Sds22, and PPP1R11 co-eluted from 14.05–15.05 ml. In each instance 0.5 ml of co-eluting fractions were collected and concentrated, then assayed by SDS-PAGE/western blot analysis.