Figure 6. PP1γ2 is inactive in chromatographically-purified fractions containing PP1γ2, Sds22, PPP1R11, and actin.
A. Protein phosphatase activity in 5-µl aliquots of DEAE-, MonoQ-, and Superose 6-purified fractions from cauda sperm protein extracts. B. SDS-PAGE/immunoblot analysis of the corresponding fractions probed with anti-Sds22, anti-PP1γ2, anti-PPP1R11, anti-PP2A, and anti-actin antibodies.