Figure 10. In PP1γ-null testis, PPP1R11 is ubiquitinated or associated with an ubiquitinated protein whose apparent size is equivalent to that of PPP1R11.
A. Western blot analysis of wild-type and PP1γ-null testis protein extracts probed with either anti-mono- and poly-ubiquitinylated conjugates or anti-PPP1R11 antibodies, as indicated below the blots. B. Western blot of anti-PPP1R11 or preimmune serum immunoprecipitates from testis extracts probed with a monoclonal antibody that recognizes mono- and poly-ubiquitinylated conjugates.