Table 2. RT-QPCR validation of microarray results/Relative level of up and down-regulated mRNA in control and NBCCS fibroblasts^*.

Gene Name	CTRL pool			missense pool			nonsense pool			average NBCCS/average CTRL	NBCCS <or> CTRL p-value
	CTRL1	CTRL2	CTRL3	NBCCS1	NBCCS7	NBCCS8	NBCCS3	NBCCS6	NBCCS10
MMP1	1	1.3	3.62	10.39	30.98	5.17	24.82	23.65	4.9	8.43	p<0.025
MMP3	1	0.89	2.32	40.98	77.08	30.01	16.18	45.77	16.45	26.84	p<0.025
COL3A1	1	0.64	0.67	0.35	0.53	0.6	0.25	0.54	0.81	0.66	p≤0.05
COL7A1	1	1.67	1.45	0.45	0.57	0.74	1.38	0.48	1.19	0.58	p≤0.05
COL11A1	1	0.11	1.99	15.93	5.62	22.91	0.02	10.93	15.73	11.47	NS
LAMA2	1	0.57	0.54	0.34	0.25	0.34	0.21	0.32	0.34	0.43	p<0.025
TNC	1	1.03	0.99	1.46	1.29	1.27	0.61	0.77	2	1.22	NS
CXCL12	1	1.13	1.22	2.46	2.77	2.48	1.77	4.24	2.31	2.39	p<0.025
MGP	1	0.01	0.18	4.67	1.38	2.65	0.27	1.28	2.11	5.19	p<0.025
ANGPTL2	1	0.98	0.93	3.32	2.09	3.21	1.16	3.78	3.67	2.95	p<0.025
ANGPTL4	1	0.8	0.79	12.24	7.2	15.1	2.08	10.18	3.75	9.77	p<0.025
FGF7	1	1.76	2.07	4.56	2.63	4.48	1.32	5.09	3.75	2.26	p≤0.05
GREM1	1	2.03	4.54	3.86	1.57	8.36	1.92	5.73	4.25	1.7	NS
SFRP2	1	0.65	0.49	1.66	0.73	4.04	1.6	2.71	4.08	3.46	p<0.025
DKK3	1	0.58	1.06	0.1	0.21	0.13	0.38	0.19	0.36	0.26	p<0.025
WNT5A	1	0.98	1.89	0.52	0.39	1.05	0.42	0.74	0.69	0.5	p≤0.05
WISP2	1	0.35	0.32	0.71	2.08	1.73	0.77	1.94	1.93	2.74	p≤0.05
ID2	1	1.26	2.92	3.39	3.65	7.26	1.38	8.27	3.2	2.62	p<0.025

^*The level of mRNA of the indicated genes was measured by RT-QPCR on the mRNAs extracted from each dermal equivalent used for the microarray assay (CTRL: n = 3; NBCCS: n = 6). Results were normalized and set to 1 for the mRNA from the dermal equivalent with control fibroblast strain 1. The ratios between the average mRNA levels in the NBCCS and the control fibroblasts and the p-value are indicated. NS: not significant (p>0.05).