Table 1. Primers.

Primer	Sequence	Constructs
LcF	GGATCCATGCCATTTGTTAATAAACAATTTAATTATAAAG	Lc, L1
LcR	CTCGAG TTATTTAGAAGTTATTATCCCTCTTACAC	Lc, L2
L1R	CTCGAG TTAAAGTGACTCCTCAAAACCAAATG	L1
L2F	GGATCCGAAGTTGATACAAATCCTCTTTTAG	L2
GS-L	GGATCCGATATCAGCCATGGCC	L1-3, L1-4
GS-R	TAA CTCGAGCACCACCACCAC	L1-1,L1-2
L1b	TTCTGGTGGTGGATTTAAATCTCCTTC	L1-1
L1c	GCAAAACAAGTTCCAGTTTCATATTATGATTC	L1-4
L1d	ATCTATTGTACTTCCACCCCAAAATGG	L1-2
L1e	ACAGAATTAAAAGTTATTGATACTAATTGTATTAATGTG	L1-3
Lc-ΔL	ATTAGTATCAATAACTTTTAATTCTGT	Lc-Δ
Lc-ΔR	CTTAATCTAGTAATAATAGGACCCT	Lc-Δ
Lc-QPDL	ACCACCTATCACATTAATACAATTAGTATCAAT	Lc-QPD
Lc-QPDR	GGTGGTAGTTATAGATCAGAA	Lc-QPD
Lc-RSL	ACCATAACTACCATCTGGTTGTATCA	Lc-RS
Lc-RSR	GGAGAAGAACTTAATCTAGTAATAATA	Lc-RS
L-chainR	TCTAGAACTGGATGGTGGGAGATGGA	F1-40 L-chain cloning
H-chainR	TCTAGAACCTCCACACACAGGAACCAGTGGATAGAC	F1-40 H-chain cloning
L-chainF3	GATATCCACCATGGAGTCACAGACTCAGGTCTTTGTA	F1-40 L-chain cloning
H-chainF3	GATATCCACCATGGCTGTCTTGGGGCTGCTCTTCT	F1-40 H-chain cloning
M13F	GTAAAACGACGGCCAG	seq. pCR4 plasmids
M13R	CAGGAAACAGCTATGAC	seq. pCR4 plasmids
pGS-F	CAAATTGATAAGTACTTGAAATCC	seq. pGS-21a plasmids
pGS-R	GCTAGTTATTGCTCAGAGG	seq. pGS-21a plasmids

Sites for restriction enzymes BamHI (GGATCC), XhoI (CTCGAG), XbaI (TCTAGA) and EcoRV (GATATC) are shown underlined. Stop codons are shown in bold, either in the 5′ to 3′ (TAA) or 3′ to 5′ (TTA) orientation. The third column indicates which peptide fragments each primer was used to construct, or where the primer was used to clone the heavy and light chain variable regions of F1-40. Primers used only for sequencing are indicated by the abbreviation “seq.”