Table 2. Mutations made in the putative F1-40 epitope region on plasmid pGS-Lc.

Vector	Light chain, from T-125 to Q-162
pGS-Lc	TELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQ
pGS-Lc-Δ	TELKVIDTN---------------LNLVIIGPSADIIQ
pGS-Lc-QPD	TELKVIDTNCINVIGGGGSYRSEELNLVIIGPSADIIQ
pGS-Lc-RS	TELKVIDTNCINVIQPDGSYGGEELNLVIIGPSADIIQ

All four vectors shown above harbored and expressed the entire BoNT/A light chain (P-1 to K-438), but only the mutated region (T-125 to Q-162) is shown here. All other amino acids within the light chain were not mutated. Dashes indicate that amino acids have been deleted. Single amino acid mutations are indicated in bold.