Figure 4.

The accumulation of PE in mitochondria depends on Gep1. (A) Overexpression of Gep2 or Cho1 allows growth of Δgep1Δphb1 cells. Δgep1Δphb1[PHB1] cells overexpressing Phb1, Gep2, or Cho1 were incubated at 30°C on media with or without 5′ fluoroorotic acid, which prevents growth of cells harboring the [PHB1] expression plasmid. (B) TLC analysis of mitochondrial phospholipids isolated from Δgep1 cells overexpressing Cho1, Ups1, or Gep2. The asterisk indicates an unidentified lipid species. (C) Gep1 and Psd2 interact genetically. Yeast cells were spotted on YP plates containing glucose (YPD) or glycerol (YPG) and incubated at 30°C or 37°C. (D) Synthetic lethal interaction of Δgep1 and Δcrd1. A diploid strain heterozygous for deletions of GEP1 and CRD1 was subjected to sporulation and tetrad dissection. Arrowheads indicate inviable double mutant progeny.