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. 2009 Feb 23;184(4):583–596. doi: 10.1083/jcb.200810189

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© 2009 Osman et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Gep1 and Ups1 regulate the phospholipid composition of mitochondrial membranes. (A and B) Phospholipid profile of mitochondria lacking Gep1-like proteins. Phospholipids were extracted from mitochondria isolated from the indicated strains (ΔΔΔ: Δgep1Δgep2Δups1) and analyzed by TLC (A) and mass spectrometry (B). Mean values ± SD obtained from at least two independent mitochondrial isolations; samples analyzed in duplicate are shown in B. Asterisks indicate unidentified lipid species. (C) Cell growth in the absence of Gep1-like proteins. Fivefold serial dilutions of the indicated cells were spotted on YPD plates. Strains were grown at 30°C. (D and E) Phospholipid profile of mitochondria in cells overexpressing Gep1-like proteins. Mitochondrial phospholipids were analyzed by TLC (D) and mass spectrometry (E). Mean values ± SD obtained from three mitochondrial isolations, each analyzed in duplicate, are shown in E. (F) Impaired cell growth upon Gep1 overexpression. Gep1-like proteins were expressed in wild-type cells from high-copy plasmids under the control of the GAL promoter. Fivefold serial dilutions of the cells were grown on synthetic media containing galactose as the carbon source (SCGal) at 30°C.