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. 2009 Feb 23;184(4):501–513. doi: 10.1083/jcb.200808042

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© 2009 Avery et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Wld^S variant proteins are stably expressed in Drosophila neurons. Three independent UAS-driven transgene insertion lines for each Wld^S variant molecule were crossed to elav-Gal4, and protein extracts from heads were assayed for expression using α-Nmnat1 antibodies. Multiple lines for each construct were found to express detectable levels of protein. The expression of each of these molecules in ORNs with OR22a-Gal4 did not lead to expression of proteins at levels detectable by Western blot analysis (not depicted). Molecular masses are shown in kilodaltons. MW, molecular weight.