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. 2009 Feb 23;184(4):515–526. doi: 10.1083/jcb.200810132

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© 2009 Hsiao and Smith

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Ligase IV is required for sister telomere fusions but not for persistent sister telomere cohesion. (A) Immunoblot analysis of extracts harvested from HTC75 cells 48 h after double infection with lentiviral tankyrase 1 shRNA and GFP shRNA or two different lentiviral DNA ligase IV shRNAs. (B) Quantification of mitotic cells with persistent sister telomere associations as measured by chromosome-specific telomere FISH using a subtelomeric 16pter probe. At least 100 mitotic cells were scored for each sample. (C) Quantification of the frequency of sister chromatid fusions as measured by telomere PNA FISH. 25 metaphase spreads were scored for each sample. (D) Schematic diagram showing the fate of sister chromatids in G2. After removal of cohesion, separated sister telomeres can form a protected structure, whereas cohered sister telomeres cannot and thus become susceptible to NHEJ.