Figure 3.

ATX3Wld^S Tg mice show a Wld^S phenotype. (A, i–iv) Semithin sections of distal sciatic nerve 5 d after lesion. (i and ii) Most axons are well preserved in Wld^S heterozygotes (i), but wild-type nerves (ii) are completely degenerated. (iii and iv) Nerves from ATX3Wld^S line 1 and line 6 show preservation similar to Wld^S heterozygotes. (v–viii) Confocal images of tibial nerve 5 d after sciatic lesion from mice crossed to YFP-H. (v) Wld^S heterozygotes maintain axon continuity. (vi) All wild-type axons are highly fragmented. (vii and viii) Many lesioned ATX3Wld^S axons maintain continuity. (B, i, iii, and iv) Semithin sections of distal sciatic nerve show preserved axons (arrowheads) 14 d after lesion in Wld^S heterozygotes (i) and ATX3Wld^S (iii and iv). (ii) Wild-type axons are completely degenerated and highly vacuolized. (v–viii) Confocal images of tibial nerves 14 d after sciatic nerve lesion in mice crossed to YFP-H. (v) Wld^S axons maintain continuity in contrast to the few remnants of the fragmented wild-type axons (vi). (vii and viii) Many axons in ATX3Wld^S were also continuous at this stringent time point. Bars: (i–iv) 20 µm; (v–viii) 50 µm.