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. 2009 Mar 19;4(3):e4936. doi: 10.1371/journal.pone.0004936

Figure 5. GluR2 protein expression.

Figure 5

To determine if increased GluR2 mRNA generates elevated protein expression, we compared subjects with a range of MMSE values. Representative immunoblots of A) homogenates and B) synaptoneurosomes, (10 µg protein per lane) were detected with antibodies for GluR2 and GAPDH. C) In controls, the increased ratio (2-fold) of synaptoneurosome (white bar) GluR2 to total GluR2 in homogenates (* p<0.0002) is an indication of synaptic protein enrichment and this ratio increases by 50% in IAD patients (p<5.8E-08). In the IAD group there is a significant increase in synaptoneurosome GluR2 protein expression compared to control (** p<6.0E-05). Homogenate GluR2 (black bar) remains constant in controls and IAD. Data are mean values ±SEM for 4 controls and 4 IAD analyzed in 5 separate experiments. The ratio of GluR2 to GAPDH is given in arbitrary units based on the integrated density value which is the sum of all pixel values after background correction (IDV).