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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 12;379(1):32–37. doi: 10.1016/j.bbrc.2008.11.150

Figure 1. Palmitate prevents NRG-1β activation of Akt without inhibiting MAPK phosphorylation or insulin-induced Akt activation.

Figure 1

NRVM were pretreated with 0.5mM palmitate for 18 hrs prior to 10ng/ml NRG-1β (A) or 100nM insulin (B) treatment for 10 min. Following lysis, cells were either analyzed by Western blot for p-Akt, p-Erk1/2, p-p38 and Akt or were subjected to immunoprecipitation (IP) with either an erbB2 or erbB4 antibody followed by Western blot for phospho-tyrosine (P-Y) content. Representative blots of 3-6 separate experiments are shown.