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. Author manuscript; available in PMC: 2010 Jan 30.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 12;379(1):32–37. doi: 10.1016/j.bbrc.2008.11.150

Figure 3. Oleate inhibits palmitate/palmitate + NRG-1β-induced apoptosis and restores NRG-1β activation of Akt.

Figure 3

A) NRVM were treated with 0.4mM palmitate with or without 0.1mM oleate and 10ng/ml NRG-1β for 24 hrs before quantifying apoptosis by flow cytometry (n=4, *p<0.01). (B) NRVM is pretreated with either 0.5mM palmitate, 0.5mM oleate or 0.4mM palmitate with 0.1mM oleate for 18 hrs prior to 10 min NRG-1β (10ng/ml) treatment. Following lysis, cells were subjected to Western blot analysis to assess phospho-Akt and phospho-ERK1/2 content. Blots are representative of 3 independent experiments.