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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Virology. 2008 Aug 30;380(2):255–263. doi: 10.1016/j.virol.2008.07.028

Figure 3.

Figure 3

Protein synthetic activity and protein phosphorylation in HCMV-infected cells treated with CA and okadaic acid (OA). (A) Mock-infected or HCMV-infected HFs (72 hpi) were treated with increasing concentrations of CA for 30 minutes followed by [35S]methionine ([100 µCi/ml] labeling for 30 minutes. De novo protein synthesis was assessed by SDS-PAGE and autoradiography (top panel) and the pattern of protein phosphorylation was determined by immunoblot analysis using a phospho-threonine (P-Thr) specific antibody (middle panel). Equivalent protein loading was confirmed by immunoblot analysis for actin (bottom panel). (B) Mock-infected HFs or HCMV-infected HFs were treated with the indicated concentration of OA for two hours beginning at 72 hpi. De novo protein synthesis (top panel) was assessed as described above after Coomassie G-250 staining (bottom panel) to document equivalent protein loading.