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. Author manuscript; available in PMC: 2009 Oct 25.
Published in final edited form as: Virology. 2008 Aug 30;380(2):255–263. doi: 10.1016/j.virol.2008.07.028

Figure 4.

Figure 4

Immediate-early and/or early viral gene expression is necessary and sufficient to upregulate cellular phosphatase levels and to inhibit the effects of CA at late times during HCMV infection. (A) Immunoblot analysis of PP1α was performed on lysates obtained at 72 hpi from mock-infected HFs, HCMV-infected HFs, HFs infected with HCMV in the presence of [45 µM] ganciclovir (GCV), or HFs infected with UV-inactivated HCMV. The membrane was stripped followed by immunoblot analysis of PP2AC. Equivalent protein loading was confirmed by immunoblot analysis of actin and the inhibitory effect of ganciclovir on HCMV late gene expression was confirmed by immunoblot analysis of pp28. (B) HFs were mock-infected or infected with HCMV in the presence and absence of GCV or with UV-inactivated HCMV. At 72 hpi, cells were mock-treated or treated with [0.1 µM] CA for 20 minutes, followed by [35S]methionine labeling for 30 minutes. De novo protein synthesis was assessed by SDS-PAGE and autoradiography (top panel) and the pattern of protein phosphorylation was determined by phospho-threonine (P-Thr)-specific immunoblot analysis (bottom panel).