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. Author manuscript; available in PMC: 2009 Dec 16.
Published in final edited form as: Mol Cell Endocrinol. 2008 Oct 4;296(1-2):1–9. doi: 10.1016/j.mce.2008.09.022

Figure 5.

Figure 5

Recruitment of ERα, changes in histone H4 acetylation (AcH4), and recruitment of p300, SRC-2, and SRC-3 on the KRT13 promoter over time in response to E2, Tam or Ral. MCF-7 cells were treated with vehicle, 10nM E2, 100nM Tam or 100nM Ral for 0, 45 min, 4 h and 24 h. ChIP assays were performed with antibody against ERα, AcH4, p300, SRC-2 or SRC-3 and the ChIP samples were analyzed by real time PCR. Recruitments on the KRT13 promoter regions P2.5, P4 and the TSS (transcription start site) are shown. Each value represents the mean from three to five separate experiments.