A representative of three biological replicates is shown. 1. KN99α 2. H99 (ade2Δ), 3&4. Independent isolates of PUGE2:ADE2, 5&6. Independent isolates of PGAL7:ADE2, 7&8. Independent isolates of PGAL1:ADE2. On YPD, the PGALX:ADE2 strains are red indicating glucose repression of ADE2 expression, resulting in accumulation of adenine biosynthetic intermediates. On YPG, the PGALX:ADE2 strains are white indicating galactose induction of ADE2 expression, which resulted in wild type phenotype.