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. Author manuscript; available in PMC: 2009 Mar 11.

Published in final edited form as: Biochemistry. 2009 Feb 3;48(4):738–743. doi: 10.1021/bi801976r

Effect of TMRM labeling on NPG binding Binding of [³H]NPG to RSO vesicles containing unlabeled and TMRM-labeled Q241C LacY (A) or Q242C LacY mutant (B) was assayed by flow dialysis at a protein concentration of 30 mg/ml as described in Materials and Methods. Only fractions 4 to 16 are shown. [³H]NPG (840 mCi/mmol) at 15 µM (final concentration) was added at fraction 1. As indicated by the arrow, TDG at 15 mM (final concentration) was added at fraction 9 to displace bound NPG. , unlabeled LacY mutants; , LacY mutants labeled with TMRM.

Inline graphic — Effect of TMRM labeling on NPG binding Binding of [³H]NPG to RSO vesicles containing unlabeled and TMRM-labeled Q241C LacY (A) or Q242C LacY mutant (B) was assayed by flow dialysis at a protein concentration of 30 mg/ml as described in Materials and Methods. Only fractions 4 to 16 are shown. [³H]NPG (840 mCi/mmol) at 15 µM (final concentration) was added at fraction 1. As indicated by the arrow, TDG at 15 mM (final concentration) was added at fraction 9 to displace bound NPG. , unlabeled LacY mutants; , LacY mutants labeled with TMRM.