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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 13;45(6):756–762. doi: 10.1016/j.freeradbiomed.2008.05.029

Figure 3.

Figure 3

Staining of aortic homogenates obtained from control, diabetic, and diabetic pigs treated with insulin with anti-SERCA C674-SO3H antibody (A), anti-SERCA IID8 antibody (B), or anti-GAPDH antibody (C). Aortic homogenate protein (30 µg in A and C, and 100 µg in B was separated by SDS-PAGE. As indicated on each blot are samples from pigs #1–8 listed in the supplemental table from the three groups of pigs. Below each lane in A and B are values of densitometry in arbitrary units integrated over each entire lane. Primary antibody concentrations were A, 1:1000; B, 3:10,000; and C, 1:1000.