Staining of aortic homogenates obtained from control, diabetic, and diabetic pigs treated with insulin with anti-SERCA C674-SO3H antibody (A), anti-SERCA IID8 antibody (B), or anti-GAPDH antibody (C). Aortic homogenate protein (30 µg in A and C, and 100 µg in B was separated by SDS-PAGE. As indicated on each blot are samples from pigs #1–8 listed in the supplemental table from the three groups of pigs. Below each lane in A and B are values of densitometry in arbitrary units integrated over each entire lane. Primary antibody concentrations were A, 1:1000; B, 3:10,000; and C, 1:1000.