Figure 4.
NESI-MS/MS spectrum of the SERCA peptide 656EFDELNPSAQREACLNARCFAR677 containing C674-SO3H. After immuno-precipitation of SERCA from aortic homogenate of diabetic pig #4, the 70 kDa gel band was cut, digested in trypsin, and analyzed by NESI-MS/MS.
* Indicates alkylation of cysteines caused by iodoacetamide present during the in-gel digestion. # Indicates the sulfonic acid modified cysteine.