. Author manuscript; available in PMC: 2009 Nov 26.

Published in final edited form as: J Am Chem Soc. 2008 Nov 26;130(47):15764–15765. doi: 10.1021/ja805555a

Table 1.

IC₅₀ values in nM of DW12, NP309, FL172, and FL411 against GSK3β, Pim1, and PAK1^a

	GSK3β	Pim1	PAK1
rac-DW12	0.32 ± 0.03	0.14 ± 0.01	1090 ± 80
rac-NP309	0.28 ± 0.04	0.17 ± 0.02	770 ± 70
Δ-FL172	440 ± 30	140 ± 23	3480 ± 220
Λ-FL172	1480 ± 60	440 ± 15	130 ± 10
Δ-FL411	1930 ± 90	470 ± 15	5200 ± 815
Λ-FL411	14400 ± 1600	2840 ± 200	110 ± 10

IC₅₀ values obtained by phosphorylation of substrates with [γ-³²P]ATP in the presence of 1 μM ATP (IC₅₀ ≈ K_i). All experiments were run in triplicate. K_m values for ATP (in μM): GSK3β = 9.3 ± 0.8, Pim1 = 48 ± 6, PAK1 = 4.4 ± 0.3.