Table 1.
ACh EC50, ANOVA, and nH for the Cys mutants
| Construct | EC50 (μm) | ANOVAa | nH | n |
|---|---|---|---|---|
| αβγδ | ||||
| wild type | 2.96 ± 0.54 | 1.30 ± 0.11 | 4 | |
| αY277C | 8.42 ± 0.72 | ** | 1.15 ± 0.04 | 3 |
| αK276C | 4.42 ± 0.46 | ns | 1.31 ± 0.02 | 3 |
| αG275C | 0.64 ± 0.03 | *** | 1.18 ± 0.06 | 3 |
| αI274C | 34.8 ± 4.5 | *** | 1.34 ± 0.02 | 3 |
| αL273C | 1.45 ± 0.15 | ns | 1.36 ± 0.03 | 3 |
| αP272C | 1.34 ± 0.07 | ns | 1.42 ± 0.06 | 3 |
| αV271C | 2.74 ± 0.17 | ns | 1.47 ± 0.21 | 3 |
| αA270C | 2.37 ± 0.60 | ns | 1.08 ± 0.14 | 4 |
| αS269C | 0.18 ± 0.03 | *** | 1.41 ± 0.06 | 4 |
| αS268C | 0.79 ± 0.13 | *** | 1.45 ± 0.08 | 5 |
| αT267C | 28.0 ± 5.2 | *** | 1.37 ± 0.07 | 5 |
| αS266C | 17.0 ± 2.9 | *** | 1.26 ± 0.08 | 3 |
| αP265C | 3.25 ± 0.43 | ns | 1.11 ± 0.04 | 3 |
| αI264C | 9.0 ± 1.6 | *** | 1.38 ± 0.05 | 8 |
| αL263C | 0.40 ± 0.06 | *** | 1.41 ± 0.05 | 4 |
| αE262C | 2.52 ± 0.40 | ns | 1.27 ± 0.06 | 3 |
The two mutants that varied most from wild type and for which the dose–response curves are shown in Figure 2 are in bold.
aOne-way ANOVA of log(EC50) with Dunnett's posttest; ns, p > 0.05, not significant; **0.001 < p < 0.01, very significant; ***p < 0.0001, extremely significant.