FIGURE 3. Effects of Sp1 and NPM on constitutive transcription.

A, expression constructs for Sp1 and NPM were co-transfected with the promoter reporter constructs, and responses by Sp1, NPM, or Sp1 plus NPM were determined. B and C, Sp1 and NPM expression constructs at different concentrations were co-transfected with the promoter reporter constructs. The increased Sp1 (in B) and NPM (in C) were measured by Western blotting, and effect of Sp1 and NPM on the reporter activity driven by different promoters was determined. The vector DNA was added to make an equal molar concentration of total DNA for each transfection. The -fold increase in promoter activities by Sp1 and NPM compared with vector only control are indicated above the histogram bars.