Chromatin from the treated and untreated cells was precipitated using NPM, TFIIB, or IgG antibody. A, the sod2 enhancer and promoter regions were analyzed by PCR. A fragment of the exon2 of the sod2 gene and the GAPDH promoter region were amplified as an untargeted control and an internal control. Additionally, IgG-precipitated product served as a negative antibody control. B, Sp1, p50, and p65 in the precipitated chromatin were quantified by Western blots normalized with IgG. Amounts of DNA fragments in A and proteins in B were normalized to the related loading controls. Indicated induction -folds were estimated by normalizing the treatments to the untreated controls.