Figure 2. Iron increases paraquat-stimulated microglial superoxide production in vitro.
(A) Microglia were treated with increasing concentrations of paraquat and 5 μM FeCl2 for 20 min. #, p < 0.01, significantly from control; *, p < 0.01, significantly from paraquat alone. (B) Time course of paraquat-stimulated superoxide production. Microglia were treated with 5 μM FeCl2, 1 μM paraquat, or 1 μM paraquat plus 5 μM FeCl2 for different time. #, p < 0.05; *, p < 0.01; @ p < 0.001, compared to the time-matched control. ##, p < 0.05; **, p < 0.05; @@ p < 0.01, compared to the time-matched paraquat alone. Mean ± S.E.M., n = 4.