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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Cell Biol. 2008 Dec 21;11(2):143–153. doi: 10.1038/ncb1819

Figure 2.

Figure 2

LRP in cerebral VSMCs is downregulated by SRF and MYOCD. (a) Multi-photon/confocal laser scanning microscopy of Cy3-Aβ42 internalization and lysosomal colocalization in control VSMCs treated with vehicle and a LRP-specific blocking antibody. Cell Tracker, blue; Cy3-Aβ42, red; Lysotracker, green. Merged images are shown (yellow-white colour is colocalization of Aβ with lysosomes). Scale bar, 20 µm. (b) LRP levels in control and AD cerebral VSMCs, and in AD VSMCs transduced with Ad.shGFP and Ad.shSRF. (c) Real-time quantitative PCR (qPCR)for LRP mRNA in AD VSMCs alone (vehicle) and transduced with Ad.shGFP or Ad.shSRF. (d) LRP levels in control VSMC transduced with Ad.GFP or Ad.MYOCD. (e) Real-time qPCR for LRP mRNA in control VSMCs alone (vehicle) and transduced with Ad.GFP or Ad.MYOCD. Data represent mean ± s.e.m. of 3 independent cultures per group (b–e).