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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Cell Biol. 2008 Dec 21;11(2):143–153. doi: 10.1038/ncb1819

Figure 4.

Figure 4

MYOCD overexpression in pial arteries suppresses focal LRP-mediated Aβ clearance in mice. (a, b) MYOCD, SRF, smooth muscle α-actin, smooth muscle calponin, SREBP2 and LRP levels determined by western blotting in cerebral pial vessels isolated after in vivo transduction with Ad.GFP or Ad.MYOCD in 5-month-old C57Bl6 mice (a). Graph shows band density of test-proteins relative to β-actin (mean ± s.e.m. from 5 animals per group, b). (c) Immunocytochemical staining for nuclear SREBP2 and cellular LRP in pial vessels after MYOCD gene transfer. LRP, red; SREBP2, green; TO-PRO3, grey. Scale bars, 20 µm. (d, e) Effects of MYOCD overexpression on endogenous Aβ42 levels in mouse transduced pial vessels (d) and focally in brain (e). Each point represents an individual animal value. Full scans of western blot data are shown in Supplementary Information, Fig. S4.