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. 2009 Jan 26;184(2):197–203. doi: 10.1083/jcb.200806047

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© 2009 Dewitt et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Excitation path-length changes caused by difference in cell thickness. The top diagram shows how cell thickness varies across the cell, which has a thin skirt around it and a thicker terminal region. The position of a confocal optical section is shown which would include the thicker terminal region but not the skirt. An example of this artifact is shown below (spreading human neutrophil), together with its intensity profile. For this and other confocal images shown in this paper, the resonant scanning head of the Leica SP2 confocal microscope with a 63x oil immersion objective NA 1.32 (HCX-PL- APO) was used