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. 2009 Jan 26;184(2):225–239. doi: 10.1083/jcb.200808049

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© 2009 Rogers et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — slimb RNAi elevates centriole number. (A) Effects of slimb RNAi are not solely via effects on cell cycle. Cells stained for D-PLP (red) and DNA (blue) were quantitated for DNA content by HTM (5,000 cells/histogram). Centrioles were manually counted in ∼150 randomly selected cells with 2C, 4C, or 8C DNA. Example cells and mean, median, and range of centriole numbers are shown below sampled histogram regions. Histograms are not shown to scale. Asterisks denote a significant difference; *, P < 0.007 (unpaired t test). Slimb or E2F1 depletion eliminates the 8C population. (B) Cell cycle progression (assessed via HTM; 5,000 cells/histogram) and centriole number were scored in day 7 RNAi-treated cells. D-PLP–labeled centrioles (red) were manually counted. Histograms are shown to scale. (C) Unique centriole configurations in Slimb-depleted cells that contained more than the mean number of D-PLP–labeled centrioles (red). White tracing marks cell borders. Insets show centrioles at a higher magnification. (A–C) Bars, 5 µm. (D–F) Transmission electron micrographs of interphase Slimb-depleted cells. (D) Typical centriolar microtubule arrangement of the centriole in cross section (red arrow). Other centrioles are sagittal sections oriented in different manners. Excess centrioles in rows (E) and clusters (F).