Table II.
Quantitation of centriole number in S phase–arrested RNAi-treated S2 cells
| Daya | Control RNAi | slimb RNAi | plk4 RNAi | plk4/slimb RNAi | ||||||||
| Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | Total | Mean ± SD | Median | |
| 0 | 159 | 2.8 ± 3.0 | 2.0 | 173 | 3.2 ± 4.0 | 2.0 | 170 | 2.9 ± 3.5 | 2.0 | 171 | 2.8 ± 3.6 | 2.0 |
| 1 | 148 | 2.6 ± 2.9 | 2.0 | 169 | 3.0 ± 3.3 | 2.0 | 175 | 2.6 ± 2.9 | 2.0 | 172 | 2.5 ± 3.2 | 2.0 |
| 2 | 181 | 2.3 ± 2.1 | 2.0 | 169 | 2.8 ± 3.1 | 2.0 | 166 | 2.8 ± 3.2 | 2.0 | 186 | 2.7 ± 2.8 | 2.0 |
| 3 | 169 | 2.5 ± 2.5 | 2.0 | 179 | 3.4 ± 3.4b | 3.0 | 170 | 2.4 ± 2.5 | 2.0 | 171 | 2.9 ± 3.8 | 2.0 |
| 4 | 157 | 2.2 ± 2.3 | 2.0 | 157 | 3.9 ± 3.8b | 3.0 | 160 | 2.6 ± 2.9 | 2.0 | 160 | 2.4 ± 3.1 | 2.0 |
| 5 | 150 | 2.4 ± 2.2 | 2.0 | 126 | 4.4 ± 3.8b | 4.0 | 142 | 2.4 ± 4.0 | 2.0 | 154 | 2.3 ± 3.3 | 2.0 |
Total, total cell number; mean and median columns refer to centriole number.
a
Cells were treated with 10 µg dsRNA, 10 µM aphidicolin, and 1.5 mM HU and processed for D-PLP immunofluorescence. This procedure was started on day 0 and repeated every 24 h.
b
Indicates a significant difference in the mean centriole value compared with the control population using an unpaired t test (P < 0.003).