Figure 8.

Impaired erythroid differentiation in N1ICD⁺ embryos. A, Representation of erythroid differentiation and marker expression. Flow cytometry was used to analyze cell surface antigens from embryos or yolk sacs at E9.5. B, CD71⁺ and Ter119⁺ populations are shown and graphed are double CD71⁺/ Ter199⁺ populations in each group. C, CD71⁺ and c-Kit⁺ populations are shown, and graphed are the percentages of CD71⁺/c-Kit⁺ double positive cells, showing increased c-Kit⁺ progenitors in N1ICD⁺ groups. D, Analysis of Flk1⁺ and c-Kit⁺ double positive population did not show significant differences but progenitors positive for Flk1 alone were significantly increased in N1ICD⁺ groups (graphed). E, Methylcellulose colony-forming assays were used to quantify BFU-E and macrophage-forming colonies from yolk sacs and embryos. Shown are representative photomicrographs of BFU-E colonies 8 days after plating. E, RT-PCR was performed using primers as indicated.