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. 2009 Mar 24;3(3):e398. doi: 10.1371/journal.pntd.0000398

Figure 8. Exogenous activation of Ov-CF-1 by FhCL1 in the presence of peptidyl-NHMec substrates.

Figure 8

(A) Initial rates of hydrolysis of the fluorogenic dipeptide substrates Z-Leu-Arg-NHMec, Z-Phe-Arg-NHMec and Z-Pro-Arg-NHMec measured by monitoring the release of the fluorogenic leaving group (−NHMec) over 150 min at 37°C. (B) Comparison of the hydrolysis of each fluorogenic peptide substrate within the linear range of the reactions (0–30 min) by recombinant Ov-CF-1 and FhCL1; relative fluorescence units are presented. The reactions included negative control (a); recombinant Ov-CF-1 alone (b); recombinant FhCL1 alone (c); Ov-CF-1 +FhCL1 (d).