Sequence alignment of the aryl acid-adenylating enzymes (AAAE) evaluated in the present work. MbtA (M. tuberculosis), YbtE (Yersinia pestis), VibE (Vibrio cholerae), DhbE (Bacillus subtilis), EntE (Escherichia coli), BasE (Acinetobacter baumanii), with Genbank accession numbers NP_216900, NP_405468, AAC45927, NP_391078, NP_415126 and CAM86025, respectively. Residues within 4 Å of 2,3-DHBAMS 5 in the respective crystal structure(47) are marked with an asterisk. Alignment was performed with ClustalW2 (28), and edited with Jalview 2.3 (29).