Table 3.

Calculated Ligand-Protein Dissociation Constants (K_D2 in nM).

Compound	4	5	18	19	20	21	22
Protein
MbtA	n.d.a	n.d.	136 ± 8	59.6 ± 4.3	(11.9 ± 0.5) × 103	> 300 × 103	(183 ± 10) × 103
YbtE	n.d.	n.d.	1.34 ± 0.29	199 ± 17	(43.7 ± 3.1) × 103	(324 ± 25) × 103	(260 ± 23) × 103
EntE	33.6 ± 6.0	n.d.	733 ± 45	696 ± 77	(211 ± 22) × 103	(8.18 ± 0.66) × 103	(125 ± 14) × 103
BasE	16.7 ± 3.1	n.d.	87.6 ± 18.2	601 ± 153	(57.5 ± 3.6) × 103	(2.63 ± 0.18) × 103	(173 ± 13) × 103
VibE	n.d.	n.d.	8.83 ± 1.29	475 ± 46	(36.6 ± 3.6) × 103	(1.64 ± 0.09) × 103	(12.0 ± 1.0) × 103
DhbE	−b	n.d.	(1.86 ± 0.22) × 103	401 ± 69	−	(3.76 ± 0.65) × 103	−

^anot determined since the displacement curve could not be fit due to the tight-binding nature of the ligand.

^bExperiment not performed.