Fig. 3.
Confirmation of linearization in alternative NF cascades. (A) Linear transformations of best fits to the dose–response curves of PGAL1-S1, PGAL1-D12, and PGAL1-T123 promoters plotted against the PGAL1-D12 promoter, based on the data from ref. 32. (B) Experimental dose–response curves and respective linear fits up to saturation levels for 3 different linearizers based on PGAL1-S1 (1 tetO2 site); PGAL1-D12 (2 tetO2 sites); and PGAL1-T123 (3 tetO2 sites). (C) Experimental dose–response curves and the corresponding linear fits up to saturation for the NF constructs with PGAL1-D12 promoter upstream and the promoters PGAL1-S1, PGAL1-D12, and PGAL1-T123 downstream, respectively.