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. 2009 Mar 11;106(13):5181–5186. doi: 10.1073/pnas.0812889106

Fig. 2.

Fig. 2.

Endogenous BAF complexes in ES cells have a distinctive subunit composition. (A) Endogenous BAF complexes were affinity purified from E14 ES cells and primary MEFs with an anti-Brg/Brm antibody (J1) and were subjected to tandem mass spectrometry. (Left) Immunoblotting of whole nuclear extracts using J1 antibody; (Right) silver stain analysis of purified complexes. (B) Summary of numbers of common and cell-type specific proteins that co-purified with Brg/Brm in ES cells, MEFs, and P0 mouse brain (neurons/neuronal progenitors). Proteins detected in all 3 samples include novel and known components of BAF complexes. A protein is considered specific to a cell type only if it has a probability of 0 in all other cell types. (C) Heatmap of the relative abundance of each BAF component across the 3 cell types, as measured by spectral quantitation. N/Np = neurons/neuronal progenitors; yellow = most abundant of 3 cell types; blue = least abundant. (D) Subunit composition of esBAF from analysis in (C). Numbers indicate BAF subunits (i.e., 155 = BAF155).