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. 2009 Mar 11;106(13):5181–5186. doi: 10.1073/pnas.0812889106

Fig. 4.

Fig. 4.

ES cells require a specific esBAF composition with respect to BAF155 and BAF170. (A) ES cells were transfected with BAF155 shRNA or pLKO control constructs. After 96 h, Oct4 protein levels, cell cycle status, and cell death were determined by flow cytometry. (B) BAF155 (155), flag-BAF170 (170), or control EV-transduced GFP+ cells were mixed with nontransduced E14 ES cells at a 1:1 ratio and were passaged every 2 d. The percentage of GFP+ cells (mean ± SD, p value from Student's t test) was determined by flow cytometry. (C) ES cells first were transduced with BAF155 transgene (Tg), BAF170 Tg, or EV controls as in (B). Stable cell lines were then transfected or transduced with control shRNA construct or shRNA construct targeting the 3′ UTR of endogenous BAF155 (BAF155KD) but not BAF155Tg. (Left) Resulting levels of BAF155 protein were normalized to Hsp90. (Right) Proliferation subsequently was assayed by BrdU incorporation (mean ± SD, indicated p-values from t test). (D) Teratomas from BAF155, BAF170, or EV-transduced GFP+ ES cells formed for 30 d in SCID mice.