Fig. 7.
Transmitted light (A–C) and sulforhodamine B (A′–C′) images of fibers after 1 and 7 days in culture. After 1 day in culture, both transmitted light (A) and sulforhodamine-stained (A′) images showed an orderly pattern of both striations and transverse (T) tubule structure. By day 7, dedifferentiating fibers exhibit various stages of disruption of T tubule structure after staining with sulforhodamine B (B′ and C′). Longitudinally oriented T tubules are apparent in C′.